4.7 Article

Coenzyme-mediated electro-grafting for ultrasensitive electrochemical DNA biosensing

期刊

SENSORS AND ACTUATORS B-CHEMICAL
卷 346, 期 -, 页码 -

出版社

ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130551

关键词

Electrochemical DNA biosensor; Coenzyme; RAFT polymerization; Grafting of polymer; Peptide nucleic acid; Signal amplification

资金

  1. National Natural Science Foundation of China [21904026]
  2. Guangzhou University [RQ2021011]
  3. Innovation Training Program for College Stu-dents of Guangzhou University [S202111078031]

向作者/读者索取更多资源

An ultrasensitive peptide nucleic acid (PNA)-based electrochemical DNA biosensor was reported using coenzyme-mediated electro-grafting of polymers for signal amplification, showing great promise in sequence-specific detection of DNA targets at ultralow concentrations.
Signal amplification through the grafting of polymers has attracted increasing attention in biodetection at ultralow concentrations. Herein, an ultrasensitive peptide nucleic acid (PNA)-based electrochemical DNA biosensor is reported by using the coenzyme-mediated electro-grafting of polymers as a novel amplification method. For the capture of DNA target, PNA is used as the probe. After hybridization, reversible addition fragmentation chain-transfer (RAFT) agents are site-specifically tethered to the deoxyribose phosphate backbone of the captured DNA targets via the carboxylate-Zr(IV)-phosphate cross-linking chemistry. Subsequently, electro-grafting of polymers through the nicotinamide adenine dinucleotide (NAD+) coenzyme-mediated electroRAFT (NAD+-eRAFT) polymerization of ferrocenylmethyl methacrylate (FcMMA) recruits numerous ferrocene redox tags for voltammetric measurement. As the electro-grafting of polymers involves simply i) the tethering of RAFT agents and ii) the NAD+-eRAFT polymerization, it is highly efficient, easy to use, and low-cost. Under optimal conditions, the voltammetric signal correlates linearly with the logarithm of DNA concentration over the range from 0.1 fM to 0.1 nM (R-2 = 0.996), with a detection limit of 0.067 fM. The PNA-based electrochemical DNA biosensor can also differentiate even single base mismatch and is applicable to DNA detection in the presence of complex serum matrices, thus showing great promise in the sequence-specific detection of DNA targets at ultralow concentrations.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据