期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 352, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130968
关键词
quantum dots; fluorescent nanofibers; electrochemiluminescence; simultaneous fluorescent and electrochemical; detection; antibody based nanobiosensor
资金
- Iran National Institute for Medical Research Development (NIMAD) [940990]
This study utilized an electrochemical sensor modified with fluorescent-immune nanofibers for the detection and determination of Streptococcus agalactiae, providing qualitative and quantitative recognition of bacteria with high specificity and sensitivity, thus enabling real-time detection in clinical samples.
In this study, detection and determination of Streptococcus agalactiae, the cause of pneumonia and meningitis in infants, were done in two steps using electrochemical sensor modified with fluorescent-immune nanofibers. In a layer by layer design, fluorescent nanofibers containing quantum dots (QDs) were electrospinned on the surface of gold electrode and functionalized by specific anti-Streptococcus agalactiae antibody. While the nanofibers were transparent to the emission of QDs, the appearance of a dark spot in the fluorescent view of the immune-sensor, qualitatively confirmed the existence of different concentrations of bacteria in the tested sample. Furthermore, the continuous electron-charge transfer system through gold-QDs-polymer chains ensured quantitative electro-chemical recognition of bacteria with a high signal-to-noise ratio. Consequently, real time detection of Strepto-coccus agalactiae was introduced by providing a detection limit of 6 CFU/mL, dynamic range of 6 order of magnetite and high specificity in clinical samples in the presence of interfering agents using fluorescent-immune sensor.
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