An integrated platform for label-free fluorescence detection and inactivation of bacteria based on boric acid functionalized Zr-MOF

Bacterial infections are a major threat to human health. The construction of a multifunctional platform that meets both the demands of sensitive detection and effective bacterial elimination is highly desirable, but still a formidable challenge. Herein, an integrated platform based on Zr-UiO-66-B(OH)(2) MOF that can achieve highly effective label-free fluorescence bacterial detection and inactivation is reported. In this design, Zr-UiO-66-B(OH)(2) is tightly linked to the surface of the bacteria attributed to the interactions between the boric acid groups of Zr-UiO-66-B(OH)(2) and the glycolipids outside the bacteria cells. Taking advantage of the fluorescence turn-on property of Zr-UiO-66-B(OH)(2) by H2O2, the bacteria could be detected with high sensitivity, with LOD of 1.0 CFU/mL. In addition, the as-obtained Zr-UiO-66-B(OH)(2) demonstrated the potential photocatalytic inactivation of bacteria under light irradiation. The photo-induced reactive oxygen species played a dominant role in the antibacterial process. This work could provide a reference for the further development of novel integrated platforms for bacterial discrimination and antibacterial applications.

Automated summary

This study presents an integrated platform based on Zr-UiO-66-B(OH)(2) MOF for highly effective label-free fluorescence bacterial detection and inactivation. The platform demonstrates the potential for further development of novel integrated platforms for bacterial discrimination and antibacterial applications.