期刊
SENSORS AND ACTUATORS A-PHYSICAL
卷 332, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.sna.2021.113070
关键词
Microfluidics; Zebrafish larva; Behavioral screening; Electric response; Habituation
资金
- Ontario Early Researcher Award, Canada [2019-0086]
- Ontario Graduate Scholarship, Canada
- National Sciences and Engineering Research Council (NSERC), Canada [RGPIN-2020-06140, RGPIN-2019-06378]
Through experiments using a microfluidic device, we found that changing the direction of electric current and voltage magnitude significantly affected the locomotion response of zebrafish larvae. Repeated exposure to electric pulses led to a diminishing response in the larvae, but the response could be fully recovered after a period of rest or introduction of a new stimulus, indicating habituation as a form of non-associative learning.
We previously showed that electric current induces zebrafish larvae to move towards the anode pole along a microchannel. For a larva with a fixed head and a moving tail, we observed that the response to electricity depended on the current magnitude. The effects of electric signal direction, voltage magnitude and habituation to repeated exposures to electric pulses were not characterized. Here, this knowledge gap was addressed by exploiting them in a microfluidic device with a head-trap to immobilize a zebrafish larva and a downstream chamber for tail movement phenotypic characterization based on response duration (RD) and tail beat frequency (TBF). We first assessed larvae's response to electric current direction (at 3 mu A) and voltage magnitude. Changing the current direction significantly altered the RD and TBF with long and low frequency responses seen when the anode was positioned at the larvae's tail. The electric voltage had a significant effect on larvae's locomotion with long RD and low TBF observed at 5.6 V in the range of 1.3-9 V. We also demonstrated that the zebrafish locomotor response to repeated 3 mu A current pulses diminished with dependency on the interstimulus interval. However, the diminished response was fully recovered after a 5-min resting period or introduction of a novel light stimulus (i.e., habituation-dishabituation strategy). Therefore, electric response suppression in zebrafish was attributed to the habituation as a form of non associative learning. Our microfluidic platform has a broad application potential in behavioral neuroscience to study cognitive phenotypes, fundamental studies on the biological roots of electric response, and in pharmacological screening. (c) 2021 Elsevier B.V. All rights reserved.
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