Fast and Sensitive Determination of Bioflavonoids Using a New Analytical System Based on Label-Free Silver Triangular Nanoplates

Optical sensors based on silver triangular nanoplates (AgTNPs) are insufficiently studied as probes for the spectrophotometric determination of biologically active compounds. In the present article, an interaction between label-free AgTNPs and bioflavonoids in the presence of silver(I) ions was assessed to outline the possibilities of AgTNPs as a colorimetric probe for the fast and sensitive determination of bioflavonoids. It is shown that the interaction was accompanied by a bathochromic shift of the local surface plasmon resonance band of nanoparticles and an increase in its intensity. Seven bioflavonoids differing in their structure were tested. The influence of the structure of analytes and the main external factors on the analytical signal is discussed in detail. It was found that the detection limits of bioflavonoids in the selected optimal conditions increased in the series morin < rutin = quercetin < taxifolin and came to 0.9, 1.2, 1.2, and 2.0 mu mol L-1, respectively. Chrysin, naringenin, and naringin were found not to affect the spectral characteristics of AgTNPs. The suggested approach was applied for the spectrophotometric determination of flavonoids in pharmaceuticals and onion peel.

Automated summary

In this study, the potential of silver triangular nanoplates (AgTNPs) as a colorimetric probe for the fast and sensitive determination of bioflavonoids was assessed. The interaction between AgTNPs and bioflavonoids in the presence of silver(I) ions resulted in a bathochromic shift and increased intensity of the nanoparticles' local surface plasmon resonance band. The detection limits of different bioflavonoids were determined and the influence of analyte structure and external factors on the analytical signal was discussed.