期刊
SCIENTIA HORTICULTURAE
卷 288, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scienta.2021.110369
关键词
Litchi; Late-ripening mutants; Anthocyanin biosynthesis; Chlorophyll degradation; Hormone signaling; Transcriptome
类别
资金
- National Key R&D Program of China [2019YFD1000900]
- National Natural Science Foundation of China [31760564, 32060659]
- Special Fund of Bagui Young Scholar of Guangxi [2019AQ01]
- Guangxi Natural Science Foundation [2017GX NSFAA198350, 2018GXNSFAA294034, 2018GXNSFAA050089]
- Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences [Guinongke2020YM02, Guinongke2020YM130, Guinongke 2021YT124, Guinongke2021YM11, Guinongke2021JM22]
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources [SKLCUSA-b201811, SKLCUSA-a201921]
- Key Laboratory Construction Project of Guangxi [20-065-39-02-07]
The study revealed that mutants with delayed coloration in litchi fruit showed delayed expression of genes involved in anthocyanin biosynthesis, chlorophyll degradation, and hormonal signaling pathways compared to the original cultivar. Transcription factors related to these processes were also identified to be different between the mutants and the original cultivar, impacting the fruit ripening program. Through the identification of novel candidate genes, significant insights were gained into the regulation of litchi fruit pigmentation and the process of litchi fruit ripening.
Litchi (Litchi chinensis Sonn.) is an important subtropical fruit crop with high economic and nutritional values. Fruit coloration is a sign of litchi fruit ripening and an important external quality traits. Two continuous generations of mutants, named MS1 and MS2, originally derived from cultivar 'Heli', exhibited the distinctive characteristic of late maturation of 20 d, with observable delays in coloration. The present study aimed to understand the mechanism underlying maturation difference between 'Heli' and its two mutants. An RNA-seq analysis revealed 6,145 differentially expressed genes, mostly identified at the color-breaking stage. The maximum expression levels of some genes involved in anthocyanin biosynthesis and chlorophyll degradation were delayed in the pericarp of the mutants compared with in the original cultivar. Generally, they peaked at earlier stages (S2/S3) in 'Heli', compared with later stages (S4/S5) in mutants. Moreover, some hormonal signal receptors, especially those involved in abscisic and ethylene signaling pathways, were also differentially expressed between the original cultivar and the mutant. Transcription factors, including MYB, bHLH, bZIP, and WRKY, correlated with anthocyanin biosynthesis, chlorophyll degradation and abscisic acid signal pathways were identified. The late pigmentation in mutants was related to the fruit ripening program, a complex and comprehensive process. Additionally, we identified novel candidate genes that are involved in the regulation of litchi fruit pigmentation of and gained significant insights into the process of litchi fruit ripening.
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