4.7 Article

Assessment of benthic invertebrate diversity and river ecological status along an urbanized gradient using environmental DNA metabarcoding and a traditional survey method

期刊

SCIENCE OF THE TOTAL ENVIRONMENT
卷 806, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.scitotenv.2021.150587

关键词

Benthic invertebrate diversity; Ecological status assessment; Environmental DNA metabarcoding; River ecosystems

资金

  1. National Natural Science Foundation of China [21976202]
  2. Major Science and Technology Program for Water Pollution Control and Treatment [2018ZX07111-002]

向作者/读者索取更多资源

Benthic invertebrate diversity is commonly used as a bioindicator for assessing aquatic ecosystem health. This study in the Jingjinji region, China, used eDNA metabarcoding and traditional methods to investigate the diversity and ecological status of benthic invertebrates and their relation to water quality along different landscapes in river systems. The results showed that eDNA metabarcoding provided more accurate and efficient data on species richness and detection of invertebrates compared to traditional methods.
Benthic invertebrate diversity is one of the most commonly used bioindicators for assessing aquatic ecosystem health in river systems. Although an increasing number of studies have focused on assessing benthic invertebrate diversity using environmental DNA metabarcoding and traditional survey methods, benthic invertebrate diver-sity and ecological status assessments performed across different landscapes within river systems have not been well documented. Here, the diversity and ecological status of benthic invertebrates and the influence of water quality on the invertebrate assemblage distribution along an urbanization gradient in rivers from the Jingjinji (JJJ) region, China, were investigated using eDNA metabarcoding and the traditional method. With the combination of the two methods, 395 benthic invertebrates from 6 phyla, 27 orders, 94 families, and 222 genera were identified. The species richness of the benthic invertebrate community in the mountain area was significantly higher than that in the urban and agricultural areas. Compared to the traditional results, eDNA metabarcoding obtained a significantly greater number of species from every sampling site (P = 0.000) and detected a notably higher abundance in Annelida (P = 0.000). Furthermore, the nonmetric multidimensional scaling (NMDS) and permutational multivariate analysis of variance (PERMANOVA) based on the Bray-Curtis dissimilarity index indicated that the benthic invertebrate communities from the different habitats were discriminated more accurately and easily using eDNA metabarcoding (P = 0.038) than with the traditional method (P = 0.829). Additionally, the assemblages identified by eDNA metabarcoding were more closely linked to water quality and could be realistically used to assess the ecological status of rivers. Our findings highlight that eDNA metabarcoding could represent a rapid and reliable method for estimating benthic invertebrate diversity and ecological status in river systems. (c) 2021 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据