Zero-valent iron mediated alleviation of methanogenesis inhibition induced by organoarsenic roxarsone

Zero-valent iron (ZVI) can enhance anaerobic digestion, and has great potential to alleviate/eliminate methanogenesis inhibition. Little is known about the feasibility of utilizing ZVI to alleviate methanogenesis inhibition that is caused by typical animal feed additive roxarsone in livestock wastewater. In this study, the role of ZVI on alleviating roxarsoneinduced methanogenic inhibition and its mechanisms were investigated. With the increase of roxarsone concentration from 5 to 50 mg/L, the inhibition of methanogenesis increased from 3.0% to 65.7%. This inhibition was alleviated by 80.7% and 57.2% when 1.0 and 10.0 g/L ZVI were added, respectively. Due to ZVI addition, an efficient arsenic immobilization onto ZVI (45.4-85.8%) was achieved mainly through the formation of FeAsO4 precipitate and adsorption by ZVI. Under the function of ZVI, hydrogenotrophic methanogenic activity was obviously restored. The microbial community analysis indicates that the ZVI-regulated alleviation on the methanogenesis inhibition was attributed to the enrichment of Methanobacterium and Methanosarcina. The findings from this study demonstrate that ZVI addition is an effective way for treatment of organoarsenic-contaminated wastewater.

Automated summary

This study investigated the role of zero-valent iron (ZVI) in alleviating methanogenesis inhibition caused by the animal feed additive roxarsone. The results showed that the addition of ZVI effectively alleviated the methanogenesis inhibition induced by roxarsone, mainly through immobilization of arsenic onto ZVI and restoration of hydrogenotrophic methanogenic activity. Microbial community analysis revealed that the alleviation of methanogenesis inhibition was attributed to the enrichment of Methanobacterium and Methanosarcina.