Combined effect of CO2 concentration and low-cost urea repletion/starvation in Chlorella vulgaris for ameliorating growth metrics, total and non-polar lipid accumulation and fatty acid composition

Different CO2 concentration such as 0.03, 5, 10 and 15% and low-cost urea repletion/starvation in Chlorella vulgaris on growth, total and non-polar lipid content and fatty acid composition was studied. Chlorella vulgaris grown at 0.03% CO2 apparently revealed inferior biomass yield 0.55 g/L on 14th day compared to CO2 supplemented cells. In the case of CO2 supply, 15% CO2 has unveiled higher biomass yield at about 1.83 g/L on day 12 whereas biomass yield for 5 and 10% CO2 supplemented cells was 1.61 and 1.73 g/L, respectively on 12th day of cultivation. The biomass productivity (g) per liter per day was 32 mg in control condition whereas it was 125, 134 and 144 mg/L/d in 5, 10 and 15% CO2 supplied cells, respectively. Lipid content of the strain grown at control, 5, 10 and 15% CO2 was 21.2, 22.1, 23.4 and 24.6%, respectively and however, without CO2 addition in low-cost urea repleted and urea depleted medium grown cells revealed 21.2 and 24.2%, respectively. Interestingly, strain grown at 15% CO2 supply in urea deplete medium yielded 28.7% lipid and contribution of non-polar lipids in total lipids is 69.7%. Further, the fatty acid composition of the strain grown in 15% CO2 supply in urea depleted medium showed C16:0, 016:1, C18:1 and 018:3 in the level of 30.12, 9.98, 23.43, and 11.97%, respectively compared to control and urea amended condition.

Automated summary

The study investigated the effects of different CO2 concentrations on the growth, lipid content, and fatty acid composition of Chlorella vulgaris. Results showed that higher CO2 levels led to increased biomass yield and lipid content, with 15% CO2 supply resulting in the highest biomass yield and lipid content.