4.7 Article

Exopolysaccharides matrix affects the process of extracellular electron transfer in electroactive biofilm

期刊

SCIENCE OF THE TOTAL ENVIRONMENT
卷 806, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.scitotenv.2021.150713

关键词

Bioelectrochemical systems; Exopolysaccharides; Geobacter sulfurreducens; Biofilm formation; Extracellular electron transfer

资金

  1. National Natural Science Founda-tion of China [42077211]
  2. National Program for Support of Top-Notch Young Professionals, Guangdong Special Support Program [2017TX04Z351]
  3. Science and Technology Program of Guangzhou, China [202002030123]

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The study investigated the role of exopolysaccharides in electroactive biofilms by constructing an exopolysaccharides-deficient Geobacter sulfurreducens-based BES. The findings demonstrated the essential role of exopolysaccharides in extracellular electron transfer (EET) and early biofilm formation, with pili playing a compensatory role in biofilm formation. The mutant biofilm had lower cell viability, thinner biofilm, and decreased electrochemical activity compared to the wild-type biofilm.
The applications of bioelectrochemical systems (BESs) in the field of environment and energy are achieved through the bioelectrocatalytic process of electroactive biofilms. As a primary component of biofilm, the role of exopolysaccharides in electroactive biofilm in BESs is poorly understood. This study constructed an exopolysaccharides-deficient Geobacter sulfurreducens-based BES to explore the role of exopolysaccharides in electroactive biofilm. Compared with the wild type, the mutant biofilm expressing less exopolysaccharides de-creased the capacity of current generation. In the mutant biofilm, the content of exopolysaccharides decreased significantly, resulting in a thinner biofilm and lower cell viability compared with the wild-type biofilm. How-ever, the mutant with overexpressed pili developed a mature biofilm with extended time, which indicating the importance of exopolysaccharides for early biofilm formation and the compensatory role of pili in biofilm for-mation. The mutant biofilm had less content of c-type cytochromes (c-Cyts) and lower electrochemical activity of extracellular polymeric substances than the wild-type biofilm, suggesting a function of exopolysaccharides an-choring extracellular c-Cyts that essential to extracellular electron transfer (EET) in electroactive biofilms. Our findings demonstrated the essential role of exopolysaccharides in the process of EET in electroactive biofilm, which contributed to a better understanding and optimization of the performance of BESs. (c) 2021 Elsevier B.V. All rights reserved.

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