Responses of simultaneous anammox and denitrification (SAD) process to nitrogen loading variation: Start-up, performance, sludge morphology and microbial community dynamics

The effects of loading variation on the efficiency, EPS, sludge morphology and microbial population of simultaneous anammox and denitrification (SAD) were thoroughly investigated with the low-abundance SAD sludge. Results indicated that the first stage lasted the longest (33d), and the average removal rate of TN can be maintained above 95%. The specific anammox activity (SAA), specific denitrification activity and PN/PS continued to increase, but the excessive loading caused the effluent to deteriorate rapidly, and SAA and PN/PS also decreased slightly, but it could be recovered quickly. The contribution rate of anammox and denitrification to N removal reached 87.6% and 12.4% eventually, respectively. The abundance of AnAOB was 10.68%-18.01%, 9.01%-15.54%, 5.74%-12.88% in the upper, middle and lower layers, respectively. Candidatus Kuenenia was always the dominant AnAOB, especially after high loading inhibition. The abundance of denitrifying bacteria (mainly Bacillus, Comamonas and Denitratisoma) gradually became the highest. (c) 2021 Published by Elsevier B.V.

Automated summary

The study found that simultaneous anammox and denitrification (SAD) system has a high contribution rate to total nitrogen removal, but excessive loading can result in deterioration of effluent quality, which can be quickly recovered. AnAOB showed different abundance levels in different layers, with Kuenenia consistently being the dominant AnAOB, while denitrifying bacteria abundance gradually increased.