Nitrogen removal through sediment denitrification in the Yangtze Estuary and its adjacent East China Sea: A nitrate limited process during summertime

Nitrogen nutrient surplus is the main cause of a series of environmental problems in the Yangtze Estuary and its adjacent East China Sea (ECS). Denitrification plays an important role in controlling nitrate dynamics and fate in estuarine and coastal environments. We investigated the natural and potential rates of denitrification in the sediments of the Yangtze Estuary and ECS via slurry incubation experiments combined with acetylene inhibition techniques to reveal its contributions to total nitrogen reduction in this hypereutrophic continental shelf area. Key environmental factors, such as the sediment grain size, sediment extractable inorganic nitrogen (NH4+, NO3- and NO2-), sediment organic carbon (SOC), total nitrogen (TN), isotopic compositions (delta C-13 and delta N-15), etc., were also investigated to determine the main factors controlling the denitrification processes. The measured rates of denitrification ranged from 0.39 to 28.49 ng N g(-1).h(-1). The total nitrogen removed by denitrification in the study area was 3.7 x 10(10) g during August. In total, at least 3.3% of the external inorganic nitrogen transported annually into the estuary could be removed by the denitrification processes in the study area. The sediment denitrification rates correlated significantly with the extractable ammonium and delta N-15 values of surface sediments, indicating that coupled nitrificationdenitrification processes may play an important role in nitrogen removal. Almost undetectable levels of nitrate in the sediment further revealed that nitrate supply, regardless of diffusion from the overlying water or production by sediment nitrification processes, is the bottleneck for denitrification. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The study found that denitrification processes in the sediments of the Yangtze Estuary and East China Sea play a significant role in reducing total nitrogen, with key environmental factors such as sediment grain size, sediment organic carbon content, and total nitrogen content controlling the process.