Beehive products as bioindicators of antimicrobial resistance contamination in the environment

The use of antimicrobials in agricultural, veterinary and medical practice exerts selective pressure on environmental microbiota, promoting the emergence and spread of antimicrobial resistance (AMR), a global concern for the One Health Initiative Task Force (OHITF). Honeybees have been studied as bioindicators of AMR in the environment, but little is known about beehive products like honey and pollen. The aim of this study was to assess the prevalence of AMR genes (ARGs) in beehive products and investigated their origins. Specifically, possible associations between ARGs, microbiota and other characteristics of different honey and pollen samples, including country of origin, flower type, type of commercial distribution and environmental factors, such as land use, weather and composition of the environment surrounding the beehives were investigated. We found that beehive products harboured ARGs conferring resistance to beta-lactams, macrolides, (fluoro)quinolones and polymyxins. Most samples possessed resistance to multiple antimicrobial classes, with honey and pollen showing similar ARG profiles. Even if Lactobacillus and Acinetobacter genera were common in the microbial communities of both honey and pollen, Bacillus, Clostridium, and Bombella defined honey microbiota, while Pseudomonas and Vibrio were enriched in pollen. ErmB and blaTEM-1 co-occurred with Lactobacillus and Fructobacillus, while positive associations between beta-lactams and macrolides and anthropogenic environments (i.e. industrial and commercial areas and non-irrigated arable lands) were found. Altogether, our findings suggest that ARGs in honey and pollen might originate from the honeybee foraging environment, and that the beehive products can be used as bioindicators of the AMR environmental contamination. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

The use of antimicrobials in agriculture, veterinary medicine, and human healthcare has led to the emergence and spread of antimicrobial resistance (AMR) in environmental microbiota. This study aimed to investigate the prevalence and origin of AMR genes (ARGs) in beehive products, such as honey and pollen. The results showed that honey and pollen samples contained ARGs conferring resistance to multiple classes of antimicrobials. The findings suggest that honey and pollen can serve as bioindicators of AMR environmental contamination and that the antimicrobial resistance in these products might originate from the honeybee's foraging environment.