期刊
RNA
卷 28, 期 5, 页码 668-682出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.078793.121
关键词
3 ' UTR; Giardia lamblia; long-read sequencing; poly(A) site
资金
- National Institutes of Health (NIH) [R35GM128680]
- RNA Bioscience Initiative
- Australian National Health and Medical Research Council L1 Investigator grant [APP1194330]
- Walter and Eliza Hall Institute of Medical Research through the Victorian State Government Operational Infrastructure Support
- Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme
- [T32 AI074491]
In G. lamblia, despite pared-down cleavage and polyadenylation machinery, 3' end formation still appears to be an important regulatory step for gene expression.
During pre-mRNA processing, the poly(A) signal is recognized by a protein complex that ensures precise cleavage and polyadenylation of the nascent transcript. The location of this cleavage event establishes the length and sequence of the 3 ' UTR of an mRNA, thus determining much of its post-transcriptional fate. Using long-read sequencing, we characterize the polyadenylation signal and related sequences surrounding Giardia lamblia cleavage sites for over 2600 genes. We find that G. lamblia uses an AGURAA poly(A) signal, which differs from the mammalian AAUAAA. We also describe how G. lamblia lacks common auxiliary elements found in other eukaryotes, along with the proteins that recognize them. Further, we identify 133 genes with evidence of alternative polyadenylation. These results suggest that despite pared-down cleavage and polyadenylation machinery, 3 ' end formation still appears to be an important regulatory step for gene expression in G. lamblia.
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