期刊
INSECT SCIENCE
卷 23, 期 3, 页码 487-499出版社
WILEY
DOI: 10.1111/1744-7917.12305
关键词
diversity; Haplopelma hainanum; tarantula venom; toxin; transcriptome
类别
资金
- National Basic Research Program of China (973 program) [2012CB114600]
- Chongqing City Fundamental and Frontier Research Program [CSTC2014JCYJA80004]
- Fundamental Research Funds for the Central Universities [XDJK2013C004]
Tarantula venoms provide a model system for studying toxin selectivity, structure-activity relationships and molecular evolution of peptide toxins. Previous studies have identified a large number of peptide toxins in the venom of the Chinese bird spider Haplopelma hainanum, generally regarded as a highly venomous spider. However, the lack of available RNA-seq transcriptomic and genomic data is an obstacle to understanding its venom at the molecular level. In this study, we investigated the venom gland transcriptome of H. hainanum by RNA-seq, in the absence of an available genomic sequence. We identified 201 potential toxins among 57 181 de novo assembled transcripts, including knottins, Kunitz-type toxins, enzymes and other proteins. We systematically identified most of the knottins and Kunitz-type toxins, some of which showed strongly biased expression in the venom gland, including members of the huwentoxin-1, huwentoxin-2 and magi-1 families. We also discovered several novel potential toxins. These data demonstrate the high molecular and structural diversity in the venom toxins of H. hainanum. This study offers a useful strategy for exploring the complex components of spider venoms.
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