期刊
INSECT MOLECULAR BIOLOGY
卷 25, 期 3, 页码 227-238出版社
WILEY
DOI: 10.1111/imb.12219
关键词
Psyllids; Bactericera cockerelli; Liberibacter; reference genes; gene expression; polymerase chain reaction
资金
- U.S. Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) [2012-67013-19431]
- NIFA [2012-67013-19431, 578723] Funding Source: Federal RePORTER
The selection of reference genes is a crucial step for quantitative real-time PCR analyses and increasingly the use of more than one reference gene for accurate and reliable normalization is being recommended. In this study, a set of six genes was selected and their stability was assessed in different life stages and female organs of Bactericera cockerelli harbouring or not the bacterial pathogen 'Candidatus Liberibacter solanacearum' (Lso) haplotype B. The stability of each gene was determined using the BESTKEEPER, NORMFINDER and GENORM programs. These analyses identified elongation factor-1a, ribosomal protein subunit L5 and ribosomal protein subunit 18 as the most stable genes to analyse gene expression during the insect life stages irrespective of Lso presence; Lso haplotype B only affected their respective ranking. By contrast, a common set of normalizers could not be found amongst the different female organs tested (bacteriomes, alimentary canals and reproductive organs).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据