期刊
INSECT MOLECULAR BIOLOGY
卷 25, 期 4, 页码 355-361出版社
WILEY
DOI: 10.1111/imb.12232
关键词
genome editing; germline transformation; pigmentation mutations; visible marker
资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) [15K14306, 26113702, 26114502, 23220007]
- Japan Society for the Promotion of Science (JSPS)
- [15J05088]
- Grants-in-Aid for Scientific Research [26113702, 15J05088, 16H06371, 26114502, 15K14306, 23220007] Funding Source: KAKEN
How behavioural diversity emerged in evolution is an unexplored subject in biology. To tackle this problem, genes and circuits for a behaviour need to be determined in different species for phylogenetic comparisons. The recently developed clustered regulatory interspaced short palindromic repeats/CRISPR associated protein9 (CRISPR/Cas9) system made such a challenge possible by providing the means to induce mutations in a gene of interest in any organism. Aiming at elucidating diversification in genetic and neural networks for courtship behaviour, we attempted to generate a genetic tool kit in Drosophila subobscura, a nonmodel species distantly related to the genetic model Drosophila melanogaster. Here we report the generation of yellow (y) and white mutations with the aid of the CRISPR/Cas9 system, and the rescue of the y mutant phenotype by germline transformation of the newly established y mutant fly line with a y(+)-marked piggyBac vector. This successful mutagenesis and transformation in D. subobscura open up an avenue for comprehensive genetic analyses of higher functions in this and other nonmodel Drosophila species, representing a key step toward systematic comparisons of genes and circuitries underlying behaviour amongst species.
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