4.6 Article

Histomorphometric and transcriptomic features characterize silk glands' development during the molt to intermolt transition process in silkworm

期刊

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 76, 期 -, 页码 95-108

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2016.07.002

关键词

Silkworm; Molt to intermolt transition; Silk gland development; Cell renewal; Histomorphology; Transcriptome

资金

  1. key program of the National Natural Science Foundation of China [31530071]
  2. National Natural Science Foundation of China [31372380]
  3. Fundamental Research Funds for the Central Universities of China [XDJK2013A026]

向作者/读者索取更多资源

The molt-intermolt cycle is an essential feature in holometabolous and hemimetabolous insects' development. In the silkworm, silk glands are under dramatic morphological and functional changes with fibroin genes' transcription being repeatedly turned off and on during the molt-intermolt cycles. However, the molecular mechanisms controlling it are still unknown. Here, silk gland's histomorphology and transcriptome analysis were used to characterize changes in its structure and gene expression patterns from molt to intermolt stages. By using section staining and transmission electron microscope, a renewable cell damage was detected in the silk gland at the molt stage, and an increased number of autophagosomes and lysosomes were found in silk gland cells' cytoplasm. Next, by using RNA sequencing, 54,578,413 reads were obtained, of which 85% were mapped to the silkworm reference genome. The expression level analysis of silk protein genes and silk gland transcription factors revealed that fibroin heavy chain, fibroin light chain, P25/fhx, sericin1, sericin3 and Dimm had consistent alteration trends in temporal expression. In addition, differentially expressed genes (DEGs) were identified, and most of the DEGs associated with ecdysone signal transduction, mRNA degradation, protein proteolysis, and autophagy were significantly down-regulated in the transition from molt to intermolt, suggesting that these pathways were activated for the silk gland renewal. These findings provide insights into the molecular mechanisms of silk gland development and silk protein genes transcriptional regulation during the molt to intermolt transition process. (C) 2016 Elsevier Ltd. All rights reserved.

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