期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 78, 期 -, 页码 50-57出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2016.09.001
关键词
Pyriproxyfen; Insecticide resistance; P450; Olyset Duo
资金
- European Union [265660 AvecNet]
- 'Francisco Jose de Caldas' PhD fellowship from the Colombian Administrative Department of Science, Technology and Innovation (COLCIENCIAS) [529]
Pyrethroid resistance is widespread in the malaria vector Anopheles gambiae leading to concerns about the future efficacy of bednets with pyrethroids as the sole active ingredient. The incorporation of pyriproxyfen (PPF), a juvenile hormone analogue, into pyrethroid treated bednets is being trialed in Africa. Pyrethroid resistance is commonly associated with elevated levels of P450 expression including CYP5 6M2, 6P2, 6P3, 6P4, 6P5, 6Z2 and 9J5. Having expressed these P450s in E. coli we find all are capable of metabolizing PPF. Inhibition of these P450s by permethrin, deltamethrin and PPF was also examined. Deltamethrin and permethrin were moderate inhibitors (IC50 1-10 mu M) of diethoxyfluorescein (DEF) activity for all P450s apart from CYP6Z2 (IC50 > 10 mu M), while PPF displayed weaker inhibition of all P450s (IC50 > 10 mu M) except CYP's 6Z2 and 6P2 (IC50 1-10 mu M). We found evidence of low levels of cross resistance between PPF and other insecticide classes by comparing the efficacy of PPF in inhibiting metamorphosis and inducing female sterility in an insecticide susceptible strain of An. gambiae and a multiple resistant strain from Cote d'Ivoire. (C) 2016 The Authors. Published by Elsevier Ltd.
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