期刊
REPRODUCTIVE TOXICOLOGY
卷 106, 期 -, 页码 115-125出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2021.10.011
关键词
Di-(2-ethylhexyl) phthalate; Prepubertal testis; Weighted gene coexpression network analysis; Bioinformatics
资金
- National Natural Science Foundation of China [82071632]
- Innovation Program for Chongqing's Overseas Returnees [cx2019030]
- Chongqing Municipal Health Commission (High-level Medical Reserved Personnel Training Project of Chongqing)
- Guangdong Basic and Applied Basic Research Foundation [2020A1515110796]
This study established a testicular injury model using different concentrations of DEHP, and identified key hub genes and pathways through WGCNA analysis to investigate the mechanism of DEHP-induced injury in the prepubertal testis, providing new insights for further understanding this mechanism.
Di-(2-ethylhexyl) phthalate (DEHP) is an environmental endocrine disruptor that accumulates in organisms in various ways and induces male reproductive system disorders. In this study, we established a testicular injury model by gavage with different concentrations of DEHP. The testes were then collected for RNA sequencing (RNA-seq), and the results were analyzed by bioinformatics and verified by experiments. Our research results show that different concentrations of DEHP interfere with testicular development differently. Weighted gene coexpression network analysis (WGCNA) generated sixteen modules and identified the turquoise module as key. Then, estrogen receptor 1 (ESR1), filamin A (Flna) and Furin were identified as hub genes. qPCR and immunohistochemistry results revealed that all three hub genes were upregulated. We detected the locations of these genes by immunohistochemistry. ESR1 was mainly located in Leydig cells; Flna immunostaining is observed in the Leydig and some germ cells and Furin staining was seen in almost all types of testicular cells. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed enrichment mainly in MAPK signaling pathways, p53 signaling pathways, HIF-1 signaling pathways, protein processing in the endoplasmic reticulum, apoptosis, the cell cycle, RNA degradation, etc. This is the first study using WGCNA to investigate the mechanism of DEHP-induced injury in the prepubertal testis, providing new research angles to further understand the mechanism of DEHP-induced injury in the prepubertal testis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据