期刊
RENEWABLE ENERGY
卷 185, 期 -, 页码 363-375出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.renene.2021.12.044
关键词
Repeated-batch fermentation; Integrated biore finery; Recombinant yeast; Lignocellulosic hydrolysate; Fixed-bed reactor; Cell immobilization
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Brasil (CAPES) [001]
- Sao Paulo Research Foundation (FAPESP) [2016/10636-8]
A strategy based on strain evaluation and integration with 1G ethanol production was used to develop a robust and scalable process for 2G ethanol production. The study found that using mixed medium of crude bagasse hydrolysate and molasses achieved high ethanol concentrations and long-term stability.
The development of a robust and scalable process for 2G ethanol production from renewable feedstock requires high ethanol titers and long-term stability. A strategy based on strain evaluation and integration with 1G ethanol production was used to overcome these challenges. Three engineered superior S. cerevisiae strains were evaluated in industrial media, using either pure sugarcane bagasse hydrolysates or mixed with molasses from sugarcane processing. Cell recycling was limited to 4 batches and decreasing productivity was observed in fermentation media based on concentrated bagasse hydrolysate, even after detoxification. When crude bagasse hydrolysate was mixed with molasses (MBH medium), fermentations with up to 10 cell recycles and final ethanol concentrations over 50 g/L were achieved with all strains. In a fixed-bed reactor operated with the selected MDS130 strain and MBH medium, remarkable ethanol productivities up to 22.8 g/L/h were reached and up to 20 cell recycles were possible.(c) 2021 Elsevier Ltd. All rights reserved.
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