期刊
PROTEOMICS
卷 22, 期 9, 页码 -出版社
WILEY
DOI: 10.1002/pmic.202100031
关键词
biolayer interferometry; mass spectrometry; molecular interaction
资金
- Gis-Ibisa
This work combines biolayer interferometry (BLI) technique with mass spectrometry (MS) to demonstrate the feasibility of BLI-MS in complex biological mixtures.
Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to identify the proteins interacting with the bait. We provide for the first time the proof of concept of the feasibility of BLI-MS in complex biological mixtures.
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