Artificial intelligence-driven phenotyping of zebrafish psychoactive drug responses

Zebrafish (Danio rerio) are rapidly emerging in biomedicine as promising tools for disease modelling and drug discovery. The use of zebrafish for neuroscience research is also growing rapidly, necessitating novel reliable and unbiased methods of neurophenotypic data collection and analyses. Here, we applied the artificial intelligence (AI) neural network-based algorithms to a large dataset of adult zebrafish locomotor tracks collected previously in a series of in vivo experiments with multiple established psychotropic drugs. We first trained AI to recognize various drugs from a wide range of psychotropic agents tested, and then confirmed prediction accuracy of trained AI by comparing several agents with known similar behavioral and pharmacological profiles. Presenting a framework for innovative neurophenotyping, this proof-of-concept study aims to improve AI-driven movement pattern classification in zebrafish, thereby fostering drug discovery and development utilizing this key model organism.

Automated summary

This study utilized AI neural network algorithms to analyze zebrafish locomotor track data, providing novel methods for neurophenotypic data collection and analysis. By training AI to recognize different psychotropic drugs and confirming its accuracy, a framework for improving movement pattern classification in zebrafish was presented, in order to advance drug discovery and development using this model organism.