Configurational fingerprints of multicellular living systems

Cells cooperate as groups to achieve structure and function at the tissue level, during which specific material characteristics emerge. Analogous to phase transitions in classical physics, transformations in the material characteristics of multicellular assemblies are essential for a variety of vital processes including morphogenesis, wound healing, and cancer. In this work, we develop configurational fingerprints of particulate and multicellular assemblies and extract volumetric and shear order parameters based on this fingerprint to quantify the system disorder. Theoretically, these two parameters form a complete and unique pair of signatures for the structural disorder of a multicellular system. The evolution of these two order parameters offers a robust and experimentally accessible way to map the phase transitions in expanding cell monolayers and during embryogenesis and invasion of epithelial spheroids.

Automated summary

Cells cooperate as groups to achieve structure and function at the tissue level, with transformations in material characteristics essential for vital processes. Configurational fingerprints and order parameters are developed to quantify system disorder, forming a complete and unique pair for structural disorder in multicellular systems. Evolution of these order parameters offers a way to map phase transitions in expanding cell monolayers and during embryogenesis and invasion of epithelial spheroids.