期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2122030119
关键词
cell adhesion; cell-cell contact formation; mechanosensing
资金
- Imaging & Optics, Electron Microscopy, and Zebrafish Facility Scientific Service Units at the Institute of Science and Technology Austria (ISTA)Nasser Darwish-Miranda
- ISTA Fellow Marie-Curie Co-funding of regional, national, and international programmes [P_IST_EU01]
- EuropeanMolecular Biology Organization Long-Term Fellowship Grant, ALTF [187-2013]
- Schroedinger Fellowship [J4332-B28]
- European Research Council Advanced Grant (MECSPEC)
The tension of the actomyosin cell cortex is crucial in determining the growth and size of cell-cell contact. This relationship increases proportionally within a certain range of tension, but inversely scales above a critical threshold. This switch is caused by cortical tension promoting the anchoring of E-cadherin to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact.
Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level of cortical tension outside of the cell-cell contact, when pulling at the contact edge, scales with the total size to which a cell-cell contact can grow [J.-L. Mai<^>tre et al., Science 338, 253-256 (2012)]. Here, we show in zebrafish primary germ-layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell-cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. After tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell-cell contact size is limited by tensionstabilizing E-cadherin-actin complexes at the contact.
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