期刊
INORGANIC CHEMISTRY
卷 55, 期 24, 页码 12544-12558出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.inorgchem.6b02357
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资金
- Nordion (Canada)
- Natural Sciences and Engineering Research Council (NSERC) of Canada (CRD)
- NSERC
- University of British Columbia
- Canadian Institutes of Health Research
- Canada Council for the Arts
- Natural Sciences and Engineering Research Council (NSERC) of Canada (Discovery)
- Natural Sciences and Engineering Research Council (NSERC) of Canada (USRA)
Three dipicolinic acid amine-derived compounds functionalized with a carboxylate (H(3)dpaa), phosphonate (H(4)dppa), and bisphosphonate (H(7)dpbpa), as well as their nonfunctionalized analogue (H(2)dpa), were successfully synthesized and characterized. The 1:1 lanthanum(III) complexes of H(2)dpa, H(3)dpaa, and H(4)dppa, the 1:2 lanthanum (III) complex of H(2)dpa, and the 1:1 gallium(III) complex of H(3)dpaa were characterized, including via X-ray crystallography for [La-4(dppa)(4)(H2O)(2)] and [Ga(dpaa)(H2O)]. H(2)dpa, H(3)dpaa, and H(4)dppa were evaluated for their thermodynamic stability with lanthanum(III) via potentiometric and either UV-vis spectrophotometric (H(3)dpaa) or NMR spectrometric (H(2)dpa and H(4)dppa) titrations, which showed that the carboxylate (H(3)dpaa) and phosphonate (H(4)dppa) containing ligands enhanced the lanthanum(III) complex stability by 3-4 orders of magnitude relative to the unfunctionalized ligand (comparing log beta(ML), and pM values) at physiological pH. In addition, potentiometric titrations with H(3)dpaa and gallium(III) were performed, which gave significantly (8 orders of magnitude) higher thermodynamic stability constants than with lanthanum(III). This was predicted to be a consequence of better size matching between the dipicolinate cavity and gallium(III), which was also evident in the aforementioned crystal structures. Because of a potential link between lanthanum(III) and osteoporosis, the ligands were tested for their bone-directing properties via a hydroxyapatite (HAP) binding assay, which showed that either a phosphonate or bisphosphonate moiety was necessary in order to elicit a chemical binding interaction with HAP. The oral activity of the ligands and their metal complexes was also assessed by experimentally measuring log P-o/w values using the shake-flask method, and these were compared to a currently prescribed osteoporosis drug (alendronate). Because of the potential therapeutic applications of the radionuclides Ga-67/68, radiolabeling studies were performed with Ga-67 and H(3)dpaa. Quantitative radiolabeling was achieved at pH 6.5 in 10 min at room temperature with concentrations as low as 10(-5) M, and human serum stability studies were undertaken.
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