期刊
POULTRY SCIENCE
卷 101, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.psj.2021.101558
关键词
transcriptome; miRNA; epididymis; rooster; sperm motility
资金
- Beijing Innovation Con-sortium of Agriculture Research System [BAIC04 -2021]
In this study, the molecular mechanisms underlying sperm motility in the epididymis of roosters were investigated. The researchers identified candidate genes and miRNAs that affect sperm motility and shed light on the regulatory mechanisms of fertility. The findings provide valuable information for understanding and improving chicken production.
Sperm motility is a crucial trait in chicken production, and the epididymis is an essential organ in the reproductive system. Currently, the molecular mechanisms underlying sperm motility in the epididymis are unclear. In this study, 8 cDNA libraries and eight miRNA libraries were constructed from roosters (4 chickens per group) with diverse sperm motility. After a comparative analysis of epididymal transcriptomes, we detected 84 differentially expressed genes (DEGs) using the edgeR package. Integrated interpretation of DEGs indicated that MMP9, SLN, WT1, PLIN1, and LRRIQ1 are the most promising candidate genes affecting sperm motility in the epididymis of roosters. MiR-146a, mir135b, and mir-205 could play important regulatory roles in sperm maturation, capacitation, and motility. Additionally, a comprehensive analysis of the mRNA and miRNAs transcriptomes in silico identified a promising gene-miRNA pair miR-135b-HPS5, which may be a vital regulator of sperm motility in the epididymis. Our find-ings provide novel integrated information of miRNAs and genes that shed light on the regulatory mechanisms of fertility in roosters.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据