期刊
POULTRY SCIENCE
卷 101, 期 4, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.psj.2022.101757
关键词
avian pathogenic Escherichia coli; response regulator; OmpR; pathogenicity
资金
- National Natural Science Foundation of China [31772707, 31972644]
- University Synergy Innova-tion Program of Anhui Province [GXXT-2019-035]
This study constructed ompR gene mutant and complement strains using the CRISPR-Cas9 system. It was found that inactivation of ompR gene attenuated the pathogenicity of APEC and affected bacterial motility, biofilm formation, and curli production.
Avian colibacillosis is a serious systemic infectious disease in poultry and caused by avian patho-genic Escherichia coli (APEC). Previous studies have shown that 2-component systems (TCSs) are involved in the pathogenicity of APEC. OmpR, a response regulator of OmpR/EnvZ TCS, plays an important role in E. coli K-12. However, whether OmpR correlates with APEC pathogenesis has not been established. In this study, we constructed an ompR gene mutant and complement strains by using the CRISPR-Cas9 system and found that the inactivation of the ompR gene attenuated bacterial motility, biofilm formation, and the production of curli. The resistance to environmental stress, serum sensitivity, adhesion, and invasion of DF-1 cells, and pathogenicity in chicks were all significantly reduced in the mutant strain AE17DompR. These phenotypes were restored in the complement strain AE17C-ompR. The qRT-PCR results showed that OmpR influences the expression of genes associated with the flagellum, biofilm formation, and virulence. These findings indicate that the regulator OmpR contributes to APEC pathogenicity by affecting the expression and function of virulence factors.
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