Effects of diet hulless barley and beta-glucanase levels on ileal digesta soluble beta-glucan molecular weight and carbohydrate fermentation in laying hens

Exogenous beta-glucanase (BGase) improves nutrient digestibility and production performance in laying hens fed barley-based diets, but the effect of enzyme and the dosage on beta-glucan depolymerization and fermentation in the gastrointestinal tract is poorly understood. The objectives of the study were to determine the effects of hulless barley (HB) and BGase levels on digestive tract beta-glucan depolymerization and fermentation in laying hens. A total of 108 Lohman-LSL Lite hens were housed in cages and fed 2 levels of HB (CDC Fibar; 0 and 73%) by substituting wheat in the diet and graded levels of BGase (Econase GT 200 P from ABVista; 0, 0.01 and 0.1% - 0, 20,000, and 200,000 BU/kg) in a 2 x 3 factorial arrangement. Birds were fed experimental diets for 8 weeks, starting at 35 wk of age. Digestive tract samples were collected at the end of the experiment. Statistical significance was set at P <= 0.05. Beta-glucan peak molecular weight was lower with the 0.1 compared to both 0 and 0.01% BGase levels, whereas weight average molecular weight was lower with the 0.1 compared to 0% BGase for 73% HB. The maximum molecular weight for the smallest 10% beta-glucan molecules decreased with the increasing BGase. Overall, beta-glucan molecular weight in the ileum was higher when the birds were given 73 in comparison to 0% HB diets. Total and major short chain fatty acids (SCFA) in the ileum were lower with 0.1 and 0.01 (except propionic acid) compared to 0% BGase in the birds fed 73% HB, but not 0% HB. Interactions between the main effects were found for the cecal acetic and isobutyric acids. In conclusion, exogenous BGase depolymerized high molecular weight beta-glucan in HB and wheat. The effects of HB and BGase on carbohydrate fermentation were not apparent, although it appears ileal SCFA concentrations were lower with increasing levels of BGase.

Automated summary

This study investigated the effects of exogenous beta-glucanase (BGase) and hulless barley (HB) on beta-glucan depolymerization and fermentation in the digestive tract of laying hens. The results showed that BGase can depolymerize high molecular weight beta-glucan in HB and wheat. The effects of HB and BGase on carbohydrate fermentation were not significant, but increasing levels of BGase appeared to decrease ileal concentrations of short chain fatty acids (SCFA).