期刊
POULTRY SCIENCE
卷 100, 期 11, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.psj.2021.101432
关键词
astrovirus; causative agent; goose gout; next-generation sequencing
资金
- Major Natural Science Research Project of Jiangsu Province, China [18KJA230001]
- Jiangsu Agricultural Science and Technology Innovation Project [CX (18)1004]
- Qing Lan Project
- Jiangsu Modern Agricultural Industrial Technology System [JATS[2020]345]
- Six Talent Peaks Project of Jiangsu Province, China [NY-00]
Recent research has shown that GAstV-1 may be one of the causative agents of goose gout in China, with significant differences from GAstV-2. This discovery helps to deepen our understanding of the evolution of astroviruses causing goose gout, and provides potential options for developing biological products to treat the disease.
In recent years, goose gout, a severe infectious disease, has affected the development of the goose industry in China. Two different genotypes of goose astrovirus (GAstV), named as GAstV-1 and GAstV-2, were identified. GAstV-2 viruses are known to be the causative agent of goose gout; however, GAstV-1 has not been isolated, and the relationship between GAstV-1 and goose gout is unknown. One full genome sequence, designated as GAstV/CHN/TZ03/2019 (TZ03), was determined from the clinical tissue samples of a diseased gosling using next-generation sequencing. The complete genome of TZ03 was 7,262 nucleotides in length with typical genomic characteristics of avastroviruses. The TZ03 strain shares the highest identity (96.6%) with the GAstV-1 strain FLX, but only 51.5 to 61.3% identity with other astroviruses in Avastrovirus. Phylogenetic analysis revealed that the TZ03 strain clustered together with the GAstV-1 strains FLX and AHDY and was highly divergent from GAstV- 2 viruses. The TZ03 strain was successfully isolated from goose embryos and caused 100% mortality of goose embryos after 5 passages. Electron microscopy showed that the virus particles were spherical with a diameter of similar to 22 nm. The clinical symptoms were reproduced by experimental infection of healthy goslings, which were similar to those caused by GAstV-2 strains. Our data show that GAstV-1 is one of the causative agents of the ongoing goose gout disease in China. These findings enrich our understanding of the evolution of GAstVs that cause gout and provide potential options for developing biological products to treat goose gout.
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