期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 184, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.postharvbio.2021.111772
关键词
Fresh-cut potato; Browning; Isoleucine; Polyphenol oxidase; Antioxidant capacity
资金
- Potato Industry Innovation Team for Modern Agricultural Industry Technology System [SDAIT-16-11]
- Tai Shan Industry Leading Talents Project, Shandong Province [LJNY201702]
The study demonstrated that isoleucine (Ile) can reduce browning in fresh-cut potatoes and improve antioxidant capacity, showing potential for use as a natural antioxidant.
Fresh-cut potatoes are susceptible to enzymatic browning. In this study, the effect of isoleucine (Ile) on the browning of fresh-cut potatoes was evaluated, and the mechanism by which Ile influenced the browning was revealed. Results showed that exogenous Ile application could reduce the browning of fresh-cut potato chips. At 2-4 degrees C, control chips had lost saleability on day 1, while 1.0 % Ile treated chips were still acceptable on day 4. Ile alleviated the decrease of tyrosine, prevented the production of brown pigments, and inhibited the browning of potato mashes. The addition of Ile to potato mash decreased polyphenol oxidase (PPO) activity, which was partially recovered after adding copper acetate. Molecular docking showed that Ile could bind with the amino acid residues of PPO. Moreover, Ile treatment increased the activity of catalase (CAT) and peroxidase (POD), improved 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) inhibition rate, and decreased the malondialdehyde (MDA) content. This is the first research to demonstrate that Ile has potential to be used as a natural anti-browning agent and its mechanisms include decreasing PPO activity by chelating Cu2+ and interacting with the PPO amino acid residues, and improving the antioxidant capacity.
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