期刊
POLYMER TESTING
卷 104, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.polymertesting.2021.107392
关键词
RGD; pH-triggered delivery; Targeted thrombolysis; Stroke; Blood-brain barrier
资金
- National Natural Science Foundation of China [51773119, 82101372]
- Shenzhen Science and Technology Innovation Commission [JCYJ20180507183036060, JCYJ20190806161409092, KJYY20180703165202011, ZDSYS20200811142600003]
- Basic scientific research operating expenses of provincial universities [SJLY2021002]
- Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province
- K. C. Wong Magna Fund in Ningbo University
The study demonstrated that treatment with uPA-Oxd-RGD had better outcomes in a rat model of middle cerebral ischemia occlusion, reducing neurological deficits and infarct volume, as well as exhibiting anti-apoptotic effects and protecting the blood-brain barrier.
It has been shown that an arginine-glycine-aspartic acid (RGD) modified pH-triggered delivery system for the urokinase-type plasminogen activator (uPA) is resistant to enzymatic degradation and improves thrombolytic ability in vitro. Herein, we aimed to compare the thrombolytic efficacies of uPA-oxidized dextran (Oxd)-RGD and uPA using a rat model of middle cerebral ischemia occlusion (MCAO) in vivo. We found that the uPA-Oxd conjugates delayed the release of active uPA after MCAO. Thus, the rats treated with uPA-Oxd-RGD showed significantly decreased neurological deficits and infarct volume compared with those of the rats treated with uPA alone after MCAO. Furthermore, the administration of uPA-Oxd-RGD attenuated blood-brain barrier disruption and downregulated matrix metalloproteinase expression while upregulating the expression of tight junction proteins. In addition, uPA-Oxd-RGD inhibited apoptosis by suppressing pro-apoptotic caspase expression. These results suggest that the administration of uPA-Oxd-RGD is a more effective intervention in an MCAO model than uPA alone and that the pH changes in the brain tissue after an ischemic stroke may be a novel thrombolytic target.
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