期刊
PLOS ONE
卷 17, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0250865
关键词
-
资金
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT (MSIT) of the Korean government [2015R1A5A2008833, 2020R1A2C2011416]
This study evaluated the impact of adipogenic transcription factors and co-factors on autophagy gene expression during adipocyte differentiation and established a model to investigate the regulatory mechanisms involved. The findings suggest a hierarchical arrangement between adipogenic transcription factors and co-factors, which collectively regulate autophagy during adipocyte differentiation.
We previously showed that some adipogenic transcription factors such as CEBPB and PPARG directly and indirectly regulate autophagy gene expression in adipogenesis. The order and effect of these events are undetermined. In this study, we modeled the gene expression, DNA-binding of transcriptional regulators, and histone modifications during adipocyte differentiation and evaluated the effect of the regulators on gene expression in terms of direction and magnitude. Then, we identified the overlap of the transcription factors and co-factors binding sites and targets. Finally, we built a chromatin state model based on the histone marks and studied their relation to the factors' binding. Adipogenic factors differentially regulated autophagy genes as part of the differentiation program. Co-regulators associated with specific transcription factors and preceded them to the regulatory regions. Transcription factors differed in the binding time and location, and their effect on expression was either localized or long-lasting. Adipogenic factors disproportionately targeted genes coding for autophagy-specific transcription factors. In sum, a hierarchical arrangement between adipogenic transcription factors and co-factors drives the regulation of autophagy during adipocyte differentiation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据