Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats

Pre-pubertal murine models of acute colitis are lacking. Magnetic resonance colonography (MRC) is a promising minimally invasive tool to assess colitis. We aimed to: 1/ Adapt a model of acute experimental colitis to pre-pubertal rats and determine whether MRC characteristics correlate with histological inflammation. 2/ Test this model by administering a diet supplemented in transforming growth factor beta 2 to reverse inflammation. Twenty-four rats were randomized at weaning to one of 3 groups: Trinitrobenzene Sulfonic Acid (TNBS) group (n = 8) fed a standard diet, that received an intra-rectal 60 mg/kg dose of TNBS-ethanol; Control group (n = 8) fed standard diet, that received a dose of intra-rectal PBS; TNBS+MODULEN group (n = 8) that received a dose of TNBS and were exclusively fed MODULEN-IBD (R) after induction of colitis. One week after induction of colitis, rats were assessed by MRC, colon histopathology and inflammation markers (Interleukin 1 beta, Tumor necrosis factor alpha, Nitric Oxide Synthase 2 and Cyclooxygenase 2). TNBS induced typical features of acute colitis on histopathology and MRC (increased colon wall thickness, increased colon intensity on T2-weighted images, target sign, ulcers). Treatment with MODULEN-IBD (R) did not reduce signs of colitis on MRC. Inflammatory marker expression did not differ among study groups.

Automated summary

The study aimed to develop a pre-pubertal model of acute colitis and evaluate the use of magnetic resonance colonography in assessing inflammation, as well as to assess the anti-inflammatory effects of a diet supplemented with transforming growth factor beta 2. The results showed that the TNBS-induced colitis model exhibited typical features of acute colitis, but treatment with MODULEN-IBD (R) did not reduce inflammation signs, and there were no significant differences in inflammatory marker expression among the study groups.