Pathway-targeting gene matrix for Drosophila gene set enrichment analysis

Gene Set Enrichment Analysis (GSEA) is a powerful algorithm to determine biased pathways between groups based on expression profiling. However, for fruit fly, a popular animal model, gene matrixes for GSEA are unavailable. This study provides the pathway-targeting gene matrixes based on Reactome and KEGG database for fruit fly. An expression profiling containing neurons or glia of fruit fly was used to validate the feasibility of the generated gene matrixes. We validated the gene matrixes and identified characteristic neuronal and glial pathways, including mRNA splicing and endocytosis. In conclusion, we generated and validated the feasibility of Reactome and KEGG gene matrix files, which may benefit future profiling studies using Drosophila.

Automated summary

This study provided pathway-targeting gene matrixes for fruit fly based on Reactome and KEGG databases, validated using expression profiling containing neurons or glia of fruit flies. The study identified characteristic neuronal and glial pathways, including mRNA splicing and endocytosis. The feasibility of Reactome and KEGG gene matrix files was generated and validated, benefiting future profiling studies using Drosophila.