Leaf wax composition and distribution of Tillandsia landbeckii reflects moisture gradient across the hyperarid Atacama Desert

In the hyperarid Atacama Desert, water availability plays a crucial role in allowing plant survival. Along with scant rainfall, marine advective fog frequently occurs along the coastal escarpment fueling isolated mono-specific patches of Tillandsia vegetation. In this study, we investigate the lipid biomarker composition of the bromeliad Tillandsia landbeckii (CAM plant) to assess structural adaptations at the molecular level as a response to extremely arid conditions. We analyzed long-chain n-alkanes and fatty acids in living specimens (n = 59) collected from the main Tillandsia dune ecosystems across a 350 km coastal transect. We found that the leaf wax composition was dominated by n-alkanes with concentrations (total average 160.8 +/- 91.4 mu g/g) up to three times higher than fatty acids (66.7 +/- 40.7 mu g/g), likely as an adaptation to the hyperarid environment. Significant differences were found in leaf wax distribution (Average Chain Length [ACL] and Carbon Preference Index [CPI]) in the northern zone relative to the central and southern zones. We found strong negative correlations between fatty acid CPI and n-alkane ACL with precipitation and surface evaporation pointing at fine-scale adaptations to low moisture availability along the coastal transect. Moreover, our data indicate that the predominance of n-alkanes is reflecting the function of the wax in preventing water loss from the leaves. The hyperarid conditions and good preservation potential of both n-alkanes and fatty acids make them ideal tracers to study late Holocene climate change in the Atacama Desert.

Automated summary

This study investigates the lipid biomarker composition of Tillandsia plants in the hyperarid Atacama Desert and reveals their structural adaptations at the molecular level to extremely arid conditions. The findings show that the leaf wax composition is dominated by n-alkanes, which help prevent water loss. Significant differences in leaf wax distribution were found among different zones along the coastal transect. The study suggests that n-alkanes and fatty acids can serve as ideal tracers for studying climate change in the Atacama Desert.