期刊
PLANT PHYSIOLOGY
卷 188, 期 2, 页码 831-845出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/plphys/kiab405
关键词
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资金
- Sumitomo Chemical Company
- Valent BioSciences
- Donald Danforth Plant Science Center
- Advanced Bioimaging Laboratory at the Donald Danforth Plant Science Center [RRID:SCR_018951]
- National Science Foundation Major Research Instrumentation grant [DBI-0116650]
- Department of Energy BER [DE SC0020348]
- U.S. Department of Energy (DOE) [DE-SC0020348] Funding Source: U.S. Department of Energy (DOE)
This study presents technical advances in using lab-based X-ray microscopy for high-resolution 3D imaging of plant samples at multiple scales. Serial imaging and improved sample preparation methods allow for the generation of sub-micron 3D volumes co-registered with lower magnification scans, providing explicit contextual reference. This method bridges the imaging gap between light and electron microscopy and can be applied to various economically and scientifically important plant systems.
Capturing complete internal anatomies of plant organs and tissues within their relevant morphological context remains a key challenge in plant science. While plant growth and development are inherently multiscale, conventional light, fluorescence, and electron microscopy platforms are typically limited to imaging of plant microstructure from small flat samples that lack a direct spatial context to, and represent only a small portion of, the relevant plant macrostructures. We demonstrate technical advances with a lab-based X-ray microscope (XRM) that bridge the imaging gap by providing multiscale high-resolution three-dimensional (3D) volumes of intact plant samples from the cell to the whole plant level. Serial imaging of a single sample is shown to provide sub-micron 3D volumes co-registered with lower magnification scans for explicit contextual reference. High-quality 3D volume data from our enhanced methods facilitate sophisticated and effective computational segmentation. Advances in sample preparation make multimodal correlative imaging workflows possible, where a single resin-embedded plant sample is scanned via XRM to generate a 3D cell-level map, and then used to identify and zoom in on sub-cellular regions of interest for high-resolution scanning electron microscopy. In total, we present the methodologies for use of XRM in the multiscale and multimodal analysis of 3D plant features using numerous economically and scientifically important plant systems. Lab-based X-ray microscopy allows high-resolution 3D imaging of intact plant samples over a wide range of sample types and sizes, filling the imaging gap between light and electron microscopy.
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