期刊
PLANT JOURNAL
卷 109, 期 5, 页码 1183-1198出版社
WILEY
DOI: 10.1111/tpj.15624
关键词
Malus; fructose content; quantitative trait locus; sorbitol dehydrogenase; promoter; transcriptional regulation
资金
- National Natural Science Foundation of China [31872043, 32102330]
- National Natural Science Foundation of Shaanxi Province [2020JC-21]
- Training Program Foundation for the Young Talents of Northwest AF University
- Earmarked Fund for the China Agriculture Research System [CARS-28]
Fructose content plays a key role in determining fruit sweetness and quality. Research has identified MdSDH2 as a major regulatory gene controlling fructose content in apples, with ABA signaling potentially involved in fructose accumulation in apple fruit.
Fructose (Fru) content is a key determinant of fruit sweetness and quality. An F1 hybrid population of the apple cultivars 'Honeycrisp' x 'Qinguan' was used to investigate the quantitative trait locus (QTL) regions and genes controlling Fru content in fruit. A stable QTL on linkage group (LG) 01 in 'Honeycrisp' was detected on the single nucleotide polymorphism (SNP) genetic linkage maps. In this region, a sorbitol dehydrogenase (SDH) gene, MdSDH2, was detected and showed promoter variations and differential expression patterns between 'Honeycrisp' and 'Qinguan' fruits as well as their hybrids. A SNP variant (A/G) in the MdSDH2 promoter region (SDH2p-491) affected the binding ability of the transcription factor MdABI3, which can affect the expression of MdSDH2. Promoter sequences with an A nucleotide at SDH2p-491 had stronger binding affinity for MdABI3 than those with a G. Among 27 domesticated apple cultivars and wild relatives, this SNP (A/G) was associated with Fru content. Our results indicate that MdSDH2 can alter Fru content as the major regulatory gene and that ABA signaling might be involved in Fru content accumulation in apple fruit.
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