Physiological and proteomic analyses reveals that brassinosteroids application improves the chilling stress tolerance of pepper seedlings

Brassinosteroids (BRs) are important in plant resistance to chilling stress. However, limited information is available regarding the specific mechanisms involved at proteomic level. We utilized the iTRAQ proteomic approach, physiological assays and information obtained from cellular ultrastructure to clarify the underlying molecular mechanism of BRs to alleviate chilling stress in pepper (Capsicum annuum L.). Foliar application of 24-epibrassinolide (EBR) improved photosynthesis and improved cell structure by presenting a distinct mesophyll cell and chloroplast with well-developed thylakoid membranes in the leaves of pepper seedlings. We identified 346 differentially expressed proteins (DEPs), including 217 up-regulated proteins and 129 down-regulated proteins in plants under chilling (Chill) and Chill + EBR treated plants. Most of the DEPs were related to multiple pathways, including photosynthesis, carbohydrate metabolism, energy metabolism, protein biosynthesis, amino acid synthesis, redox and stress defence (ascorbate peroxidase, glutathione peroxidase and superoxide dismutase). Up-regulated DEPs were associated with the photosynthetic electron transfer chain, oxidative phosphorylation, GSH metabolism pathway, Calvin cycle and signaling pathway. The physiochemical analysis showed that EBR treatment improved the tolerance of pepper seedlings to chilling stress.

Automated summary

The study used iTRAQ proteomic approach to demonstrate the positive impact of 24-epibrassinolide on the physiological metabolism of pepper seedlings under chilling stress, improving plant tolerance to cold stress by enhancing photosynthesis and altering cell structure. This was achieved by analyzing 346 differentially expressed proteins (DEPs) related to various pathways, including photosynthesis, metabolism, and stress defense mechanisms.