4.7 Article

An In Vitro Co-Culture System for Rapid Differential Response to Fusarium oxysporum f. sp. vasinfectum Race 4 in Three Cotton Cultivars

期刊

PLANT DISEASE
卷 106, 期 3, 页码 990-995

出版社

AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PDIS-08-21-1743-RE

关键词

co-culture; cotton; FOV4; Fusarium wilt; Gossypium; in vitro

资金

  1. Cotton Incorporated [19-860]
  2. South Carolina Cotton Board [2013171]
  3. U.S. Department of Agriculture National Institute of Food and Agriculture [SC-1700530, SC-1700591]
  4. Agriculture and Food Research Initiative of the U.S. Department of Agriculture from the Critical Agricultural Research and Extension Program [2018-09116]

向作者/读者索取更多资源

An in vitro coculture system using an inert polyphenolic foam substrate and liquid medium was designed to study the resistance of domesticated cotton to Fusarium oxysporum f. sp. vasinfectum race 4. The results showed that susceptible cultivars exhibited more severe disease symptoms and plant mortality, while resistant cultivars performed better.
Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4) is a devastating fungus pathogen that causes Fusarium wilt in both domesticated cotton species, Gossypium hirsutum (Upland) and G. barbadense (Pima). Greenhouse and field-based pathogenicity assays can be a challenge because of nonuniform inoculum levels, the presence of endophytes, and varying environmental factors. Therefore, an in vitro coculture system was designed to support the growth of both domesticated cotton species and FOV4 via an inert polyphenolic foam substrate with a liquid medium. A Fusarium wilt-susceptible Pima cotton cultivar, G. barbadense 'GB1031'; a highly resistant Pima cotton cultivar, G. barbadense 'DP348RF'; and a susceptible Upland cotton cultivar, G. hirsutum 'TM-1', were evaluated for 30 days during coculture with FOV4 in this foam-based system. Thirty days after inoculation, disease symptoms were more severe in both susceptible cultivars, which displayed higher percentages of foliar damage, and greater plant mortality than observed in 'DP348RF', the resistant Pima cotton cultivar. This foam-based in vitro system may be useful for screening cotton germplasm for resistance to a variety of fungus pathogens and may facilitate the study of biotic interactions in domesticated cotton species under controlled environmental conditions.

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