☆ 4.7 Article

Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals

PLANT CELL REPORTS (2022)

Genome editing for plant research and crop improvement

Xiangqiang Zhan et al.

Summary: The CRISPR technology has significantly impacted plant biology and crop improvement, with developments ranging from gene disruption to precise editing techniques like base editing and gene targeting. Advances have also been made in using different CRISPR systems for gene transcription and epigenome control.

JOURNAL OF INTEGRATIVE PLANT BIOLOGY (2021)

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Article Plant Sciences

Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters

Chong Ren et al.

Summary: This study identified multiple effective promoters in grapevine and achieved multiplex genome editing by optimizing the CRISPR/Cas9 system, leading to increased editing efficiency. Simultaneous editing of TMT1 and TMT2 genes resulted in reduced sugar levels in grapes, highlighting the role of these genes in sugar accumulation. The findings provide valuable materials for the optimization of CRISPR/Cas9 system and expand the toolbox of grape genome editing for basic research and molecular breeding.

HORTICULTURE RESEARCH (2021)

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Article Plant Sciences

High-throughput detection and screening of plants modified by gene editing using quantitative real-time polymerase chain reaction

Cheng Peng et al.

PLANT JOURNAL (2018)

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Article Biochemical Research Methods

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Lu Long et al.

PLANT METHODS (2018)

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Review Biotechnology & Applied Microbiology

Applications and potential of genome editing in crop improvement

Yi Zhang et al.

GENOME BIOLOGY (2018)

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Article Biochemistry & Molecular Biology

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

Wenzhi Jiang et al.

NUCLEIC ACIDS RESEARCH (2013)

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Article Multidisciplinary Sciences

Whole-genome sequencing reveals untapped genetic potential in Africa's indigenous cereal crop sorghum

Emma S. Mace et al.

NATURE COMMUNICATIONS (2013)

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Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals

相关参考文献

Genome editing for plant research and crop improvement

Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters

High-throughput detection and screening of plants modified by gene editing using quantitative real-time polymerase chain reaction

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Applications and potential of genome editing in crop improvement

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

Whole-genome sequencing reveals untapped genetic potential in Africa's indigenous cereal crop sorghum

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