Cyclosporin A alleviates trophoblast apoptosis and senescence by promoting autophagy in preeclampsia

Introduction: Abnormal extravillous trophoblast (EVT) function is closely related to preeclampsia (PE) and may be caused by inadequate autophagy, apoptosis, and senescence. Cyclosporin A (CsA) is an effective immunosuppressant that has been reported to stimulate autophagy and exert benign biological effects on EVTs. Therefore, we hypothesized that CsA may display therapeutic efficacy against PE by activating autophagy. Methods: We established the nitro-L-arginine methyl ester (L-NAME)-induced preeclamptic mice model and a hypoxia-reoxygenation (H/R) model in vitro. The effects of CsA on autophagy were evaluated by western blotting (WB). The effects of CsA on apoptosis were analyzed by Hematoxylin-eosin (H&E) staining, cell apoptosis assay and WB. Senescence-associated beta-galactosidase (SA-beta-gal) staining, RT-qPCR and WB were used to examine the senescence level. RT-qPCR were used to detect the senescence-associated secretory phenotype (SASP) level. DCFH-DA fluorescent probe, dihydroethidium (DHE) staining and mitochondrial membrane potential (Delta Psi m) were used to detect senescence-associated mitochondrial dysfunction (SAMD). Results: CsA alleviated PE-like symptoms and reduced placental necrosis and senescence in mice injected with L-NAME. CsA ameliorated placental SASP and SAMD level induced by L-NAME. CsA also upregulated the expression of autophagic proteins in mouse placentas disrupted using L-NAME. In vitro, we found that CsA reversed H/R-induced apoptosis and senescence, as well as decreasing SASP and SAMD levels and upregulating autophagic proteins levels. Notably, 3-methyladenine (3-MA), an early phase inhibitor of autophagosome formation, abolished the protective effects of CsA against H/R. Discussion: CsA may display some therapeutic effects against PE by activating autophagy in vivo and in vitro.

Automated summary

This study found that CsA may have therapeutic effects against preeclampsia by activating autophagy. The results showed that CsA alleviated PE-like symptoms, reduced placental necrosis and senescence, and upregulated autophagic protein levels.