期刊
PHARMACEUTICAL BIOLOGY
卷 60, 期 1, 页码 175-184出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2021.2021948
关键词
Platelet aggregation; thromboxane; aspirin; haemorrhage; andrographolide
资金
- Health Commission of Jiangxi Province
This study demonstrates that Dehydroandrographolide succinate (DAS) has an antithrombotic effect and positively affects platelet aggregation, coagulation function, and coagulation factor activity.
Context Dehydroandrographolide succinate (DAS) is mainly used in the clinical treatment of various infectious diseases. Its potential effects on platelet aggregation and blood coagulation systems have not been reported systematically. Objective To explore whether DAS exerts an antithrombotic effect and its internal mechanism. Materials and methods Human blood samples and Sprague-Dawley (SD) rats divided into control, aspirin (30 mg/kg), and DAS groups (200, 400 and 600 mg/kg) were used to measure the platelet aggregation rate, coagulation function, coagulation factor activity, and contents of thromboxane B-2 (TXB2) and 6-keto-prostaglandin F-1 alpha (6-keto-PGF(1 alpha)). The histopathology of the SD rat gastric mucosa was also observed. All rats were administered intragastric or intraperitoneal injections once a day for 3 consecutive days. Results Compared to control group, DAS significantly inhibited the platelet aggregation rate (ED50 = 386.9 mg/kg) by decreasing TXB2 levels (1531.95 +/- 649.90 pg/mL to 511.08 +/- 411.82 pg/mL) and activating antithrombin III (AT-III) (103.22 +/- 16.22% to 146.46 +/- 8.96%) (p < 0.05). In addition, DAS significantly enhanced the coagulation factors FV (304.12 +/- 79.65% to 443.44 +/- 75.04%), FVII (324.19 +/- 48.03% to 790.66 +/- 225.56%), FVIII (524.79 +/- 115.47% to 679.92 +/- 143.34%), FX (34.90 +/- 7.40% to 102.76 +/- 29.41%) and FXI (38.12 +/- 10.33% to 65.47 +/- 34.08%), increased the content of Fg (2.18 +/- 0.39 to 3.61 +/- 0.37 g/L), shorten the PT (10.42 +/- 0.44 to 9.22 +/- 0.21 s), APTT (16.43 +/- 1.4 to 14.07 +/- 0.75 s) and TT time (37.04 +/- 2.13 to 32.68 +/- 1.29 s) (p < 0.05), while the aspirin group showed no such effect on these items but showed reduced activity of FII (89.21 +/- 21.72% to 61.83 +/- 8.95%) and FVIII (524.79 +/- 115.47% to 306.60 +/- 29.96%) (p < 0.05). Histopathological changes showed aspirin-induced gastric mucosa haemorrhage and the protective effect of DAS in the gastric mucosa. Conclusions DAS is more suitable than aspirin in thromboprophylaxis treatment, which provides a reliable theoretical and experimental basis for its clinical application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据