4.6 Article

FAEE exerts a protective effect against osteoporosis by regulating the MAPK signalling pathway

期刊

PHARMACEUTICAL BIOLOGY
卷 60, 期 1, 页码 467-478

出版社

TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2022.2039216

关键词

Ferulic acid ethyl ester; postmenopausal osteoporosis; osteoclast

资金

  1. Yunnan provincial key programs of Yunnan Eco-friendly Food International Cooperation Research Centre project [2019ZG00904, 2019ZG00909]
  2. Science and Technology Plan Project of Yunnan Province [2018IA060]
  3. Yunnan Provincial Science and Technology Project [2018FG001-035]

向作者/读者索取更多资源

Ferulic acid ethyl ester (FAEE) can inhibit osteoclastogenesis and relieve ovariectomy-induced osteoporosis through the MAPK signaling pathway. In vitro experiments showed that FAEE suppressed RANKL-induced osteoclast formation by reducing the expression of osteoclast-specific genes, proteins, and MAPK pathway-related proteins. In ovariectomized rats, FAEE maintained normal calcium balance, increased estradiol levels, and reduced bone density and mineral content.
Context Ferulic acid ethyl ester (FAEE) is abundant in Ligusticum chuanxiong Hort. (Apiaceae) and grains, and possesses diverse biological activities; but the effects of FAEE on osteoporosis has not been reported. Objective This study investigated whether FAEE can attenuate osteoclastogenesis and relieve ovariectomy-induced osteoporosis via attenuating mitogen-activated protein kinase (MAPK). Materials and methods We stimulated RAW 264.7 cells with receptor activator of NF-kappa B ligand (RANKL) followed by FAEE. The roles of FAEE in osteoclast production and osteogenic resorption of mature osteoclasts were evaluated by tartrate resistant acid phosphatase (TRAP) staining, expression of osteoclast-specific genes, proteins and MAPK. Ovariectomized (OVX) female Sprague-Dawley rats were administered FAEE (20 mg/kg/day) for 12 weeks to explore its potential in vivo, and then histology was undertaken in combination with cytokines analyses. Results FAEE suppressed RANKL-induced osteoclast formation (96 +/- 0.88 vs. 15 +/- 1.68) by suppressing the expression of osteoclast-specific genes, proteins and MAPK signalling pathway related proteins (p-ERK/ERK, p-JNK/JNK and p-P38/P38) in vitro. In addition, OVX rats exposed to FAEE maintained their normal calcium (Ca) (2.72 +/- 0.02 vs. 2.63 +/- 0.03, p < 0.05) balance, increased oestradiol levels (498.3 +/- 9.43 vs. 398.7 +/- 22.06, p < 0.05), simultaneously reduced levels of bone mineral density (BMD) (0.159 +/- 0.0016 vs. 0.153 +/- 0.0025, p < 0.05) and bone mineral content (BMC) (0.8 +/- 0.0158 vs. 0.68 +/- 0.0291, p < 0.01). Discussion and conclusions These findings suggested that FAEE could be used to ameliorate osteoporosis by the MAPK signalling pathway, suggesting that FAEE could be a potential therapeutic candidate for osteoporosis.

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