4.7 Article

Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast

期刊

PEST MANAGEMENT SCIENCE
卷 78, 期 3, 页码 1048-1059

出版社

JOHN WILEY & SONS LTD
DOI: 10.1002/ps.6716

关键词

pest management; pheromone; mating disruption; (Z; E)-9; 12-tetradecadienyl acetate; Saccharomyces cerevisiae; bioproduction of pheromone

资金

  1. King Saud University [KACST-NSTIP 12-AGR2554-02]
  2. Swedish Research Council [2018-05973]

向作者/读者索取更多资源

This study successfully demonstrated the biological production of ZETA pheromone in yeast through metabolic engineering, using genes such as E12 fatty acyl desaturase.
BACKGROUND (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses. CONCLUSION The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.

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