期刊
PARASITES & VECTORS
卷 15, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13071-021-05140-3
关键词
Toxoplasma gondii; RNA-seq; lncRNA; mRNA; Immune response Background
资金
- Agricultural Science and Technology Innovation Program (ASTIP) of China [CAAS-ASTIP-2016-LVRI-03]
- Yunnan Expert Workstation [202005AF150041]
- Veterinary Public Health Innovation Team of Yunnan Province [202105AE160014]
- Fund for Shanxi 1331 Project [20211331-13]
- Special Research Fund of Shanxi Agricultural University for High-level Talents [2021XG001]
This study revealed the transcriptional changes during Toxoplasma gondii infection in human cells through RNA sequencing, and identified differentially expressed lncRNAs and mRNAs. Functional enrichment analysis showed that T. gondii infection affected genes involved in host immune response, receptor signaling, disease, and metabolism. These results provide novel information for further research on the role of lncRNAs in immune regulation during T. gondii infection.
Background: Long non-coding RNAs (lncRNAs) are important regulators of various biological and pathological processes, in particular the inflammatory response by modulating the transcriptional control of inflammatory genes. However, the role of lncRNAs in regulating the immune and inflammatory responses during infection with the protozoan parasite Toxoplasma gondii remains largely unknown. Methods: We performed a longitudinal RNA sequencing analysis of human foreskin fibroblast (HFF) cells infected by T. gondii to identify differentially expressed long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs), and dysregulated pathways over the course of T. gondii lytic cycle. The transcriptome data were validated by qRT-PCR. Results: RNA sequencing revealed significant transcriptional changes in the infected HFFs. A total of 697, 1234, 1499, 873, 1466, 561, 676 and 716 differentially expressed lncRNAs (DEIncRNAs), and 636, 1266, 1843, 2303, 3022, 1757, 3088 and 2531 differentially expressed mRNAs (DEmRNAs) were identified at 1.5, 3, 6, 9, 12, 24, 36 and 48 h post-infection, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DElncRNAs and DEmRNAs revealed that T. gondii infection altered the expression of genes involved in the regulation of host immune response (e.g., cytokine-cytokine receptor interaction), receptor signaling (e.g., NOD-like receptor signaling pathway), disease (e.g., Alzheimer's disease), and metabolism (e.g., fatty acid degradation). Conclusions: These results provide novel information for further research on the role of lncRNAs in immune regulation of T. gondii infection.
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