期刊
PARASITES & VECTORS
卷 14, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13071-021-05100-x
关键词
Amastigote; Clinical isolate; In vitro test; Promastigote
资金
- Conselho Nacional de Pesquisa e Desenvolvimento (CNPq) [309862/2015-9]
- Professional Education Expansion Program (Proep) [442055/2019-6]
- Instituto Carlos Chagas
- Fundacao Oswaldo Cruz (FIOCRUZ)
The study showed that treating a dog with Miltefosine and allopurinol resulted in increased resistance of L. infantum to Miltefosine and amphotericin B, as well as an increase in metacyclogenesis rate. Further research with a larger sample size of L. infantum strains is recommended.
Background: Leishmania infantum is the most important etiological agent of visceral leishmaniasis in the Americas and Mediterranean region, and the dog is the main host. Miltefosine was authorized to treat canine leishmaniasis (CanL) in Brazil in 2017, but there is a persistent fear of the emergence of parasites resistant not only to this drug but, through cross-resistance mechanisms, also to meglumine antimoniate and amphotericin B. Additionally, the literature shows that acquisition of resistance is followed by increased parasite fitness, with higher rates of proliferation, infectivity and metacyclogenesis, which are drivers of parasite virulence. In this context, the aim of this study was to analyze the impact of treating a dog with miltefosine and allopurinol on the generation of parasites resistant to miltefosine, amphotericin B and meglumine antimoniate. Methods: In vitro susceptibility tests were conducted against miltefosine, amphotericin B and meglumine antimoniate with T0 (parasites isolated from a dog before treatment with miltefosine plus allopurinol),T1 (after 1 course of treatment) and T2 (after 2 courses of treatment) isolates. The rates of cell proliferation, infectivity and metacyclogenesis of the isolates were also evaluated. Results: The results indicate a gradual increase in parasite resistance to miltefosine and amphotericin B with increasing the number of treatment courses. An increasing trend in the metacyclogenesis rate of the parasites was also observed as drug resistance increased. Conclusion: The data indicates an increased L. infantum resistance to miltefosine and amphotericin B after the treatment of a dog with miltefosine plus allopurinol. Further studies with a larger number of L. infantum strains isolated from dogs with varied immune response profiles and undergoing different treatment regimes, are advocated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据