Ift88 regulates enamel formation via involving Shh signaling

Objectives The ciliopathies are a wide spectrum of human diseases, which are caused by perturbations in the function of primary cilia. Tooth enamel anomalies are often seen in ciliopathy patients; however, the role of primary cilia in enamel formation remains unclear. Materials and Methods We examined mice with epithelial conditional deletion of the ciliary protein, Ift88, (Ift88(fl)(/)(fl);K14Cre). Results Ift88(fl)(/)(fl);K14Cre mice showed premature abrasion in molars. A pattern of enamel rods which is determined at secretory stage, was disorganized in Ift88 mutant molars. Many amelogenesis-related molecules expressing at the secretory stage, including amelogenin and ameloblastin, enamelin, showed significant downregulation in Ift88 mutant molar tooth germs. Shh signaling is essential for amelogenesis, which was found to be downregulated in Ift88 mutant molar at the secretory stage. Application of Shh signaling agonist at the secretory stage partially rescued enamel anomalies in Ift88 mutant mice. Conclusion Findings in the present study indicate that the function of the primary cilia via Ift88 is critical for the secretory stage of amelogenesis through involving Shh signaling.

Automated summary

The present study suggests that the function of primary cilia, mediated by Ift88, is critical for the secretory stage of amelogenesis, involving the Shh signaling pathway.