期刊
OPTICS EXPRESS
卷 29, 期 23, 页码 38206-38220出版社
Optica Publishing Group
DOI: 10.1364/OE.434848
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资金
- European Commission [101016787, CZ.1.05/2.1.00/01.0017]
- Akademie Ved CeskeRepubliky [RVO:68081731]
- European Research Council [724530]
- Ministerstvo Skolstvi, Mladeze a Telovychovy [CZ.02.1.01/0.0/0.0/15_003/0000476, LO1212]
- European Research Council (ERC) [724530] Funding Source: European Research Council (ERC)
Imaging geometries utilizing wavefront-shaping to control light transport through a multi-mode optical fibre enable high resolution laser-scanning fluorescence microscopy inside living tissues, but are often contaminated by stray optical signals. By post-processing resulting images using intensity records of all contaminated foci, imaging performance can be significantly enhanced.
In imaging geometries, which employ wavefront-shaping to control the light transport through a multi-mode optical fibre (MMF), this terminal hair-thin optical component acts as a minimally invasive objective lens, enabling high resolution laser-scanning fluorescence microscopy inside living tissues at depths hardly accessible by any other light-based technique. Even in the most advanced systems, the diffraction-limited foci scanning the object across the focal plane are contaminated by a stray optical signal carrying typically few tens of % of the total optical power. The stray illumination takes the shape of a randomised but reproducible speckle, and is unique for each position of the focus. We experimentally demonstrate that the performance of imaging a fluorescent object can be significantly improved, when resulting images are computationally post-processed, utilising records of intensities of all speckle-contaminated foci used in the imaging procedure. We present two algorithms based on a regularised iterative inversion and regularised direct pseudo-inversion respectively which lead to enhancement of the image contrast and resolution. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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