Exosomal circCARM1 from spheroids reprograms cell metabolism by regulating PFKFB2 in breast cancer

Cancer stem cells (CSC) are the major obstacle for cancer therapy in clinic. Exosomes are one type of vesicles that containing circular RNA (circRNAs) involved in cell-cell communication. However, the roles of breast CSC (BCSC) exosomes are still unclear, and the purpose of the study was to investigate breast cancer cell metabolism reprogramming by circRNAs from BCSC exosomes. The circRNA array was performed in the exosomes secreted from spheroids of MDA-231 cells. circCARM1 was higher in BCSC exosomes than it in the parent breast cancer cells. Further investigation demonstrated that BCSC exosomes circCARM1 played an important role in breast cancer cell glycolysis by miR-1252-5p/PFKFB2. In a conclusion, BCSC exosome-derived circCARM1 played an important role in breast cancer cell glycolysis by sponging miR-1252-5p which regulated PFKFB2 expression.

Automated summary

This study investigates the role of circular RNA (circRNAs) from breast cancer stem cell (BCSC) exosomes in breast cancer cell metabolism reprogramming. The results show that circCARM1 in BCSC exosomes plays an important role in breast cancer cell glycolysis through miR-1252-5p/PFKFB2 regulation.